doi:10.1016/S0888-7543(02)00028-9    
Copyright © 2003 Elsevier Science (USA). All rights reserved.

Regular article

Radiation hybrid map, physical map, and low-pass genomic sequence of the canine prcd region on CFA9 and comparative mapping with the syntenic region on human chromosome 17small star, filled, low

D. J. Sidjanina, B. Millera, J. Kijasa, J. McElweea, J. Pillardyb, J. Malekc, G. Paic, T. Feldblyumc, C. Fraserc, G. Aclanda and G. AguirreCorresponding Author Contact Information, E-mail The Corresponding Author, a

a James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
b Computational Biology Service Unit, Cornell Theory Center, Cornell University, Ithaca, NY 14853, USA
c The Institute for Genomic Research, Rockville, MD 20850, USA

Received 19 August 2002;  accepted 8 November 2002. ; Available online 22 February 2003.
 

Abstract

Progressive rod–cone degeneration (prcd) is a canine retinal disease that maps to the centromeric end of CFA9 in a region of synteny with the distal part of HSA17q. As such, prcd has been postulated as the only animal model of RP17, a human retinitis pigmentosa locus that maps to 17q22. In an effort to establish more detailed regions of synteny between dog CFA9 and the HSA17q–ter region, we created a robust gene-enriched CFA9-RH083000 map with 34 gene-based markers and 12 microsatellites, with the highest resolution and number of markers for the centromeric end of CFA9. Furthermore, we built an approximately 1.5-Mb physical map containing both GRB2 and GALK1, genes so far identified by meiotic linkage analysis as being closest to the prcd locus, and generated about 1.2 Mb low-pass (3.2×) canine sequence. Canine to human comparative sequence analysis identified 49 transcripts that had been previously mapped to the HSA17q25 region. The generated low-pass canine sequence was annotated with a working draft of human sequence from HSA17q25, and we used this scaffold to order and orient the canine sequence against human. This order and orientation are preliminary, as high-throughput genomic sequencing of HSA17q–ter has not been fully completed.

Author Keywords: Bacterial artificial chromosome; Contig mapping; Dog; Genome sequencing; Nucleotide sequence; Physical mapping; Progressive retinal atrophy; Progressive rod–cone degeneration; Radiation hybrid mapping; Retinitis pigmentosa; Rod photoreceptors; Synteny