Project 4. Chloroplast Subproteomes and their Protein-Protein Interactions in
Arabidopsis thaliana and response to the environment
It widely recognized that the chloroplast contains a large number of unidentified proteins which are involved in different metabolic functions and in the biogenesis, maintenance and adaptation of the chloroplast (refs #27, 42). To systematically identify these proteins and their protein-protein interaction, and to follow their up- and down regulation under different abiotic conditions, we are using a proteomics approach, involving 1- and 2-Dimensional electrophoresis, off-line chromatography, different stable isotope labeling techniques, followed by matrix-assisted laser desorption/ionization time-of-flight MS (MALDI-TOF MS), on-line nano-LC electrospray ionization tandem MS (nano-LC ESI-MS/MS) and bioinformatics (refs #20, 22, 24, 28, 35, 36, 38, 43, 48). Currently, we are particularly interested in the contribution of the ascorbate (Vitamine C) defense system and in the role of plastoglobules in protecting the chloroplast from light-induced damage.
